Ye Jing, Xu Yi-kai, Liu Xian, Lü Guo-shi
Diagnostic Imaging Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Feb;26(2):139-43.
To investigate the effect of streptavidin (SA)-DTPA-Gd after intraperitoneal and intravenous administration for tumor enhancement in targeted magnetic resonance imaging (MRI).
Biotinylated monoclonal antibody CL3 (600 microg) was intravenously injected into 12 BALB/c nude mice with subcutaneous inoculation of LoVo cells, followed by administration of 80 microg avidin as the chaser 24 h later and then SA-DTPA-Gd was injected intravenously or intraperitoneally after another 30 min. MRI was performed before and 20, 60 min and 3, 6, 9, 12 h after the injection of the contrast agents, and the MR signal intensity of the tumor and liver was determined.
The maximum enhancement ratio of the tumor was 70.2% in the intravenous injection group and 46.4% in the intraperitoneal group, showing significant difference between them. The maximal enhancement rate of the liver was 23.7% in the intraperitoneal group and 20.4% in the intravenous group, showing no significant difference.
MR targeted imaging with biotinylated monoclonal antibody CL3 and SA-DTPA-Gd has specific enhancement effect. Higher blood level of SA-DTPA-Gd in the intravenous group facilitates the tumor enhancement in MRI in subcutaneous tumor model.
研究链霉亲和素（SA）-二乙三胺五乙酸钆（DTPA-Gd）经腹腔和静脉注射后在靶向磁共振成像（MRI）中对肿瘤增强的作用。
将生物素化单克隆抗体CL3（600微克）静脉注射到12只皮下接种了LoVo细胞的BALB/c裸鼠体内，24小时后给予80微克抗生物素蛋白作为追踪剂，再过30分钟后经静脉或腹腔注射SA-DTPA-Gd。在注射造影剂前以及注射后20、60分钟和3、6、9、12小时进行MRI检查，测定肿瘤和肝脏的磁共振信号强度。
静脉注射组肿瘤的最大增强率为70.2%，腹腔注射组为46.4%，两组间差异显著。腹腔注射组肝脏的最大增强率为23.7%，静脉注射组为20.4%，差异无统计学意义。
生物素化单克隆抗体CL3和SA-DTPA-Gd的磁共振靶向成像具有特异性增强作用。静脉注射组中SA-DTPA-Gd的较高血药浓度有利于皮下肿瘤模型MRI中的肿瘤增强。
