Jiang Yao-dong, Zheng Shao-bin, Wang Zhan-hui
Department of Urology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2004 Jan;24(1):99-101.
To evaluate the reliability of reverse transcription (RT)-PCR in detecting the expression of the carbonic anhydrase IX (MN/CAIX) gene in renal cell carcinoma (RCC).
MN/CAIX was examined in 5 cases of renal cell carcinoma tissues and 5 normal renal tissues. Primers specific for MN/CAIX was designed and two-step RT-PCR of the extracted total RNA performed. The products was examined by 2% agarose electrophoresis and identified by sequence analysis.
Specific bands of MN/CAIX were found in RT-PCR products of 4 case of RCC tissues, whereas could not be seen in normal renal tissues.
RT-PCR is reliable for detecting the expression of MN/CAIX gene in RCC.
