[Detection of carbonic anhydrase IX gene expression in renal cell carcinoma with reverse transcription-PCR].

OBJECTIVE

To evaluate the reliability of reverse transcription (RT)-PCR in detecting the expression of the carbonic anhydrase IX (MN/CAIX) gene in renal cell carcinoma (RCC).

METHODS

MN/CAIX was examined in 5 cases of renal cell carcinoma tissues and 5 normal renal tissues. Primers specific for MN/CAIX was designed and two-step RT-PCR of the extracted total RNA performed. The products was examined by 2% agarose electrophoresis and identified by sequence analysis.

RESULTS

Specific bands of MN/CAIX were found in RT-PCR products of 4 case of RCC tissues, whereas could not be seen in normal renal tissues.

CONCLUSION

RT-PCR is reliable for detecting the expression of MN/CAIX gene in RCC.