Schaefer Brian C, Kappler John W, Kupfer Abraham, Marrack Philippa
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):1004-9. doi: 10.1073/pnas.0307858100. Epub 2004 Jan 14.
The central zone of the supramolecular activation cluster (c-SMAC) is a zone of T cell receptor (TCR) enrichment that forms at a T cell/antigen-presenting cell (APC) junction in response to antigen stimulation. We demonstrate that there is a surprisingly complex relocalization process that brings PKC and Bcl10, two intermediates in TCR activation of NF-kappaB, to the cytoplasmic face of the c-SMAC. TCR activation causes enrichment of PKC at the c-SMAC, followed by Bcl10 relocalization to punctate cytoplasmic structures, often at sites distant from the c-SMAC. These Bcl10 structures then undergo further relocalization, becoming enriched at the c-SMAC. TCR activation of NF-kappaB therefore involves the dynamic relocalization of multiple signaling intermediates, with distinct phases proximal to and distant from the c-SMAC.
超分子激活簇的中心区（c-SMAC）是一个T细胞受体（TCR）富集区，它在T细胞/抗原呈递细胞（APC）连接处因抗原刺激而形成。我们证明，存在一个惊人复杂的重新定位过程，该过程将PKC和Bcl10这两个NF-κB的TCR激活中间产物带到c-SMAC的细胞质面。TCR激活导致PKC在c-SMAC富集，随后Bcl10重新定位到点状细胞质结构，这些结构通常位于远离c-SMAC的位点。然后这些Bcl10结构会进一步重新定位，在c-SMAC处富集。因此，NF-κB的TCR激活涉及多种信号中间产物的动态重新定位，在靠近和远离c-SMAC的区域有不同阶段。
