Yang Ming-Feng, Sun Bao-Liang, Xia Zuo-Li, Zhu Liang-Zhen, Qiu Ping-Ming, Zhang Su-Ming
Institute of Microcirculation, Affiliated Hospital of Taishan Medical College, Taian, Shandong 271000, China.
Clin Hemorheol Microcirc. 2003;29(3-4):437-43.
Decreased levels of nitric oxide play a role in the development of cerebral ischemia secondary to subarachnoid hemorrhage (SAH). The protective effect of L-arginine on brain edema following SAH was investigated in this study. Rats were divided randomly into a sham-operated, a SAH+saline group and a SAH+L-arginine group. At different time points, brain water content was determined using the wet and dry weight compared method. Brain sodium content, potassium content and calcium content were detected using an atomic absorption spectral photometer. Somatosensory evoked potentials (SEP) were also detected. It was found that rat SAH models were successfully replicated. In the SAH+saline group, brain water and sodium content were significantly higher at 6 h and 24 h than those in the sham-operated group, while brain potassium content was statistically lower than that in the sham-operated group. Brain calcium content increased from 1 h to 24 h after induction of SAH. SEP latency progressively delayed. In the SAH+L-arginine group, increases in brain water content, sodium content and calcium content, as well as decreases in brain potassium content, were not as obvious as in the SAH+saline group. L-arginine partly prevented a delay in SEP latency. In conclusion, L-arginine, a substrate of nitric oxide synthesis, may relieve brain edema in rats with experimental SAH.
一氧化氮水平降低在蛛网膜下腔出血（SAH）继发的脑缺血发展中起作用。本研究探讨了L-精氨酸对SAH后脑水肿的保护作用。将大鼠随机分为假手术组、SAH+生理盐水组和SAH+L-精氨酸组。在不同时间点，采用干湿重比较法测定脑含水量。用原子吸收光谱光度计检测脑钠含量、钾含量和钙含量。还检测了体感诱发电位（SEP）。结果发现成功复制了大鼠SAH模型。在SAH+生理盐水组，6小时和24小时时脑含水量和钠含量显著高于假手术组，而脑钾含量在统计学上低于假手术组。SAH诱导后1小时至24小时脑钙含量增加。SEP潜伏期逐渐延迟。在SAH+L-精氨酸组，脑含水量、钠含量和钙含量的增加以及脑钾含量的降低不如SAH+生理盐水组明显。L-精氨酸部分预防了SEP潜伏期的延迟。总之，一氧化氮合成的底物L-精氨酸可能减轻实验性SAH大鼠的脑水肿。
