Shen Ge Zhi, Wang Xin Qi, Yin Li Qing, Wang Jiang, Li Lin, Zhang Jing Liu
Crop Institute, Shanghai Academy of Agriculture Sciences, Shanghai 201106.
Shi Yan Sheng Wu Xue Bao. 2003 Dec;36(6):459-64.
A rolled-leaf mutant was obtained in a T-DNA(containing bar gene and Ds element) insertion population, which consist of transgenic japonica rice Zhonghua 11 mediated by Agrobacterium tumefaciens. Through self-hybridization of three generations, one of trait-purified mutants (R1-A2) was obtained and used as parent to cross with variety Zhonghua 11. The leaves of 36 F1 plants investigated were rolled and resistant to herbicide Basta. Among 852 F2 plants, the segregation ratio of rolled leaves to normal leaves(645:207) was consistent with 3:1. All rolled-leaf plants were resistant to herbicide Basta, and all normal leaf plants were sensitive to herbicide Basta. These results showed that the trait of rolled-leaf is co-segregated with Basta resistance. The total DNA of 45 rolled-leaf plants and 30 normal leaf plants in F2 population were amplified to test the presence of T-DNA by Ds primers. The results showed that the positive band were amplified in all rolled-leaf plants, but not in every normal leaf plant. In F1B1 progenies, all plants which derived from backcross parent R1-A2 were rolled leaves; while variety Zhonghua 11 was used as backcross parent, the segregation ratio of rolled-leaf to normal leaf was consistent with 1:1. Taking these data together, it indicated that the rolled-leaf mutant was co-segregation with T-DNA and controlled by single dominant gene.
在一个由农杆菌介导的转基因粳稻中花11组成的T-DNA（含有bar基因和Ds元件）插入群体中获得了一个卷叶突变体。通过三代自交，获得了一个性状纯化的突变体（R1-A2），并将其作为亲本与中花11品种杂交。对36株F1植株的叶片进行观察，发现叶片卷曲且对除草剂Basta具有抗性。在852株F2植株中，卷叶与正常叶的分离比例（645:207）符合3:1。所有卷叶植株都对除草剂Basta具有抗性，而所有正常叶植株对除草剂Basta敏感。这些结果表明，卷叶性状与Basta抗性共分离。用Ds引物对F2群体中45株卷叶植株和30株正常叶植株的总DNA进行扩增，以检测T-DNA的存在。结果表明，所有卷叶植株都扩增出了阳性条带，而不是每株正常叶植株都能扩增出阳性条带。在F1B1后代中，所有来自回交亲本R1-A2的植株都是卷叶；而以中花11品种作为回交亲本时，卷叶与正常叶的分离比例符合1:1。综合这些数据表明，卷叶突变体与T-DNA共分离，且受单显性基因控制。
