Zhu Z G, Xiao H, Fu Y P, Hu G C, Yu Y H, Si H M, Zhang J L, Sun Z X
Key Laboratory for Rice Biology, Ministry of Agriculture, P. R. China National Rice Research Institute, Hangzhou 331006, China.
Sheng Wu Gong Cheng Xue Bao. 2001 May;17(3):288-92.
An efficient and rapid gene transformation system of rice mediated by Agrobacterium tumefaciens was used. Calli induced from immature and mature embryos of Zhonghua No. 11, a japonic rice variety, were cultured with the A. tumefaciens strain EHA105 harboring the superbinary plasmid pDsBar1300 or pUBITs separately, and more than 400 independent transgenic lines inserted Ds element or Ac fragment were obtained. Some visible mutants in T0 or T1 generation were found, consisting of disease resistance, albino, dwarf, male sterile, chlorosis, early heading, late heading, stripe, etc. From the phenotype analysis, a few mutants such as dwarf and male sterile seemed to be linked to the Basta resistance and the transposon.
采用了一种由根癌农杆菌介导的高效快速的水稻基因转化系统。以粳稻品种中花11号的未成熟胚和成熟胚诱导的愈伤组织，分别与携带超级双元质粒pDsBar1300或pUBITs的根癌农杆菌菌株EHA105共培养，获得了400多个插入Ds元件或Ac片段的独立转基因株系。在T0或T1代中发现了一些可见突变体，包括抗病、白化、矮化、雄性不育、黄化、早抽穗、晚抽穗、条纹等。从表型分析来看，一些突变体如矮化和雄性不育似乎与Basta抗性和转座子有关。
