Wang Tai Xia, Li Jing Yuan, Shen Zong Gen, Hu Zheng Hai
College of Life Sciences, Northwest University, Xi'an 710069.
Shi Yan Sheng Wu Xue Bao. 2003 Oct;36(5):361-7.
The development of aloin cells and its relationship with the accumulation of anthraquinone in aloe leaf were investigated with the methods of paraffin section, semi-thin section, histochemistry and fluorescent microscopy. The results showed: cells rounded the procambium bundle differentiated into bundle sheath at the initial stage of procambium bundle developing into vascular bundle. When the sieve tube members appeared in protophloem, there were a lay of procambium bundle cells reserved between the sieve tube members and bundle sheath. These cells began to devise, then developed into aloin cells through enlargement of volume and vacuolization with the differentiation of metaphloem and metaxylem. So the aloin cells were special phloem parenchyma cells because they shared the same origin with the other phloem cells. The investigation of histochemistry reflected that there were aloin precipitate in the central vacuole of aloin cells after the material was soaked in the liquid of 1% lead acetate [Pb (CH3COO)2]. In addition, the yellow fluorescence was observed in aloin cells when the section of fresh material was investigated under the fluorescent microscope with blue light, which suggested the aloin cells of vascular bundles were the mainly storage site of anthraquinone.
采用石蜡切片、半薄切片、组织化学和荧光显微镜技术，对芦荟叶中芦荟素细胞的发育及其与蒽醌积累的关系进行了研究。结果表明：在原形成层束发育为维管束的初期，围绕原形成层束的细胞分化为维管束鞘。当原生韧皮部出现筛管分子时，在筛管分子和维管束鞘之间保留一层原形成层束细胞。这些细胞开始分化，随着后生韧皮部和后生木质部的分化，通过细胞体积增大和液泡化发育成芦荟素细胞。因此，芦荟素细胞是特殊的韧皮部薄壁细胞，因为它们与其他韧皮部细胞具有相同的起源。组织化学研究表明，材料用1%醋酸铅[Pb(CH3COO)2]溶液浸泡后，芦荟素细胞的中央液泡中有芦荟素沉淀。此外，用蓝光在荧光显微镜下观察新鲜材料切片时，在芦荟素细胞中观察到黄色荧光，这表明维管束中的芦荟素细胞是蒽醌的主要储存部位。
