del Castillo B, Alvarez-Builla J, Martinez-Honduvilla C J
Ital J Biochem. 1977 Nov-Dec;26(6):437-43.
Crude analytical abscisic acid (ABA) exhibited fluorescence (lambdaex 340, lambdaem 425 nm). The results demonstrated a relationship between fluorescence response and ABA concentration, in the range of 0.04-0.38 microgram/ml. Chromatographic purification increased the emission signal, and oxidation induced by adding a sulphuric acid: ethanol (1:1) mixture also increased and displaced it (lambdaex 450, lambdaem 495 nm). Using these properties, a fluorimetric method for identification and evaluation of ABA, had been developed, and used for inhibitor recognition in P. pinea seed coats.
粗分析脱落酸（ABA）呈现荧光（激发波长340，发射波长425纳米）。结果表明，在0.04 - 0.38微克/毫升范围内，荧光响应与ABA浓度之间存在关系。色谱纯化增强了发射信号，添加硫酸：乙醇（1:1）混合物诱导的氧化也增强并改变了发射信号（激发波长450，发射波长495纳米）。利用这些特性，已开发出一种用于鉴定和评估ABA的荧光法，并用于识别欧洲松种子皮中的抑制剂。
