采用高效液相色谱法和气液色谱法分析3-吲哚乙酸和脱落酸。

Analysis of 3-indolylacetic acid and abscisic acid by high-performance liquid chromatography and gas-liquid chromatography.

作者信息

Rodriguez A, Sanchez Tames R S

出版信息

Anal Biochem. 1985 Apr;146(1):184-90. doi: 10.1016/0003-2697(85)90414-2.

Abstract

A method of analysis of 3-indolylacetic acid (IAA) and abscisic acid (ABA), allowing the simultaneous extraction of both regulators from plant material, has been developed. The method involves extraction with methanol, isolation of the acid fraction, diazomethane methylation, separation of the hormones through reverse-phase preparative high-performance liquid chromatography, and quantification of both compounds by gas-liquid chromatography. The recovery percentage at each step was monitored with radioactive compounds added at the beginning of the process. The final recovery was 70% for IAA and 96% for ABA. The method was applied to the analysis of the IAA and ABA content of stems of hazel (Corylus avellana L.).

摘要

已开发出一种分析3-吲哚乙酸（IAA）和脱落酸（ABA）的方法，该方法可从植物材料中同时提取这两种调节物质。该方法包括用甲醇提取、酸馏分的分离、重氮甲烷甲基化、通过反相制备高效液相色谱法分离激素以及用气液色谱法对两种化合物进行定量。在该过程开始时加入放射性化合物来监测每个步骤的回收率。IAA的最终回收率为70%，ABA为96%。该方法应用于榛树（欧洲榛，Corylus avellana L.）茎中IAA和ABA含量的分析。