用于免疫扩散试验的巴西副球孢子菌外抗原的简易生产方法。
Simplified method for producing Paracoccidioides brasiliensis exoantigens for use in immunodiffusion tests.
作者信息
Camargo Z P, Berzaghi R, Amaral C C, Silva S H M
机构信息
Disciplina de Biologia Celular, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brasil.
出版信息
Med Mycol. 2003 Dec;41(6):539-42. doi: 10.1080/13693780310001615358.
A simplified method to produce Paracoccidioides brasiliensis exoantigens for immunodiffusion testing is proposed. It uses technology accessible for small laboratories with few resources in Latin America, where paracoccidioidomycosis is endemic. This procedure may replace the more complex procedure, originally proposed by Camargo et al. in 1988, that is currently commonly used. It is based on the production of exoantigen by P. brasiliensis isolate B339, a good secretor of the characteristic 43000-Da glycoprotein gp43.
本文提出了一种用于免疫扩散试验的巴西副球孢子菌外抗原的简化生产方法。该方法采用了拉丁美洲资源匮乏的小型实验室也可获得的技术，在拉丁美洲，副球孢子菌病是地方病。该程序可能会取代目前常用的、最初由卡马戈等人于1988年提出的更为复杂的程序。它基于巴西副球孢子菌分离株B339产生外抗原，B339是特征性43000道尔顿糖蛋白gp43的良好分泌者。
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