通过非同源末端连接修复DNA双链断裂

Repair of DNA double strand breaks by non-homologous end joining.

作者信息

Lees-Miller S P, Meek K

机构信息

Cancer Biology Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive, NW, Calgary, Alta., Canada T2N 4N1.

出版信息

Biochimie. 2003 Nov;85(11):1161-73. doi: 10.1016/j.biochi.2003.10.011.

DOI:10.1016/j.biochi.2003.10.011

PMID:14726021

Abstract

DNA double strand breaks (DSB) are the most serious form of DNA damage. If not repaired they can lead to cell death. If misrepaired DSBs contribute to chromosomal aberrations and genomic instability. Non-homologous end joining (NHEJ) is one of two major pathways for the repair of DSBs in human cells. Proteins known to be required for NHEJ include the DNA-dependent protein kinase (DNA-PK), XRCC4, DNA ligase IV, and Artemis. This review discusses how these and other accessory proteins may function in the repair of DSBs produced by ionizing radiation (IR) and by V(D)J recombination.

摘要

DNA双链断裂（DSB）是最严重的DNA损伤形式。如果不进行修复，它们会导致细胞死亡。如果修复错误，DSB会导致染色体畸变和基因组不稳定。非同源末端连接（NHEJ）是人类细胞中DSB修复的两种主要途径之一。已知NHEJ所需的蛋白质包括DNA依赖性蛋白激酶（DNA-PK）、XRCC4、DNA连接酶IV和Artemis。本综述讨论了这些及其他辅助蛋白如何在电离辐射（IR）和V（D）J重组产生的DSB修复中发挥作用。