Hirata Raphael, Souza Sonia M S, Rocha-de-Souza Cláudio M, Andrade Arnaldo F B, Monteiro-Leal Luiz H, Formiga Luiz C D, Mattos-Guaraldi Ana L
Disciplina de Microbiologia e Imunologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, 3 degrees andar. Vila Isabel, Av. 28 de Setembro, 87 Fundos, CEP 20 551-030, Rio de Janeiro, RJ, Brazil.
Microb Pathog. 2004 Mar;36(3):125-30. doi: 10.1016/j.micpath.2003.10.002.
Corynebacterium diphtheriae strains displayed different degrees of attachment to HEp-2 cell monolayers with two distinct adherence patterns, termed localised (LA) and diffuse (DA). The LA phenotype predominated over the DA phenotype. The non-sucrose fermenting strains expressing DA pattern adhered mostly with high index values (> or =10bact/cell). Low adhesion index (<10bact/cell) was mainly observed among sucrose fermenting strains. The fluorescein isothiocyanate (FITC)-labelled phalloidin assay (fluorescent-actin staining test) showed positive results for microorganisms of both LA and DA phenotypes. The FITC-labelled C. diphtheriae non-fimbrial surface proteins 67-72p interacted directly with HEp-2 cell membranes. Therefore, toxigenic C. diphtheriae exhibited LA and DA adherence patterns and ability to induce actin polymerisation. The experimental evidences also pointed to 67-72p as putative adhesins of C. diphtheriae to HEp-2 cells.
白喉棒状杆菌菌株对人喉癌细胞（HEp-2）单层细胞表现出不同程度的附着，呈现出两种不同的黏附模式，即局部黏附（LA）和弥漫性黏附（DA）。LA表型占主导地位。表达DA模式的非蔗糖发酵菌株大多以高指数值（≥10个细菌/细胞）附着。低黏附指数（<10个细菌/细胞）主要见于蔗糖发酵菌株。异硫氰酸荧光素（FITC）标记的鬼笔环肽检测（荧光肌动蛋白染色试验）显示LA和DA表型的微生物均呈阳性结果。FITC标记的白喉棒状杆菌非菌毛表面蛋白67 - 72p与HEp-2细胞膜直接相互作用。因此，产毒白喉棒状杆菌表现出LA和DA黏附模式以及诱导肌动蛋白聚合的能力。实验证据还表明67 - 72p是白喉棒状杆菌与HEp-2细胞的假定黏附素。
