Mattos-Guaraldi A L, Formiga L C, Andrade A F
Disciplina de Microbiologia e Imunologia, Faculdade de Ciências Médicas, Universidade Estado do Rio de Janeiro. Av. 28 de Setembro, 87-Fundos, 3 degrees andar, Vila Isabel, RJ, Brazil CEP 20.551-030.
Curr Microbiol. 1999 Jan;38(1):37-42. doi: 10.1007/pl00006769.
Corynebacterium diphtheriae strains expressed variation in hydrophobic characteristics dependent on the method used. Results of single assays are not a reliable representation of C. diphtheriae hydrophobicity. All 12 strains adhered to polystyrene surfaces; three showed spontaneous aggregation (SA) in Trypticase Soy Broth (TSB) medium, and eight exhibited autoagglutination in phosphate-buffered saline (PBS; AA-positive). The salt aggregation test (SAT) values </=0.002 or >/=1.6 represented breakpoints for groups of strains with differing hydrophobicity. C. diphtheriae strains showed affinity towards n-hexadecane. Percentages of adhesion varied from 31% to 63% and were not directly related to morphological n-hexadecane adhesion patterns. Diffuse and localized adhesion patterns were noted predominantly among sucrose-positive and sucrose-negative strains, respectively. Strains of the sucrose-negative biotype expressed a higher degree of hydrophobicity. The choice of the growth medium influenced the hydrophobicity, not the hemagglutinating activity (HA) of C. diphtheriae. Heating bacterial suspensions at 121 degrees C decreased both HA and hydrophobicity of three strains. However, hydrophobins and hemagglutinins were trypsin and detergent resistant. The treatment of microorganisms with Clostridium perfringens neuraminidase increased the hydrophobicity but not the HA titers of strains tested. Hemagglutinins were partially responsible for hydrophobicity. Hydrophilic AA-negative strains adhered strongly to glass but expressed weak HA. Sialylglycoconjugates functioned as hydrophilins on C. diphtheriae surfaces.
白喉棒状杆菌菌株表现出的疏水特性因所采用的方法而异。单次检测结果并不能可靠地反映白喉棒状杆菌的疏水性。所有12株菌株均能黏附于聚苯乙烯表面；3株在胰蛋白酶大豆肉汤（TSB）培养基中表现出自发聚集（SA），8株在磷酸盐缓冲盐水（PBS；AA阳性）中表现出自凝现象。盐聚集试验（SAT）值≤0.002或≥1.6代表不同疏水性菌株组的断点。白喉棒状杆菌菌株对正十六烷有亲和力。黏附百分比在31%至63%之间变化，且与正十六烷的形态学黏附模式无直接关系。弥漫性和局限性黏附模式分别主要见于蔗糖阳性和蔗糖阴性菌株。蔗糖阴性生物型的菌株表现出更高程度的疏水性。生长培养基的选择影响白喉棒状杆菌的疏水性，但不影响其血凝活性（HA）。将细菌悬液在121℃加热会降低3株菌株的HA和疏水性。然而，疏水蛋白和血凝素对胰蛋白酶和去污剂具有抗性。用产气荚膜梭菌神经氨酸酶处理微生物会增加受试菌株的疏水性，但不会提高其HA效价。血凝素部分介导了疏水性。亲水性AA阴性菌株能强烈黏附于玻璃，但HA表达较弱。唾液酸糖缀合物在白喉棒状杆菌表面起亲水性物质的作用。
