[大鼠阴茎海绵体组织中一氧化氮合酶同工酶I和III的蛋白质及mRNA表达]
[Protein and mRNA expression of nitric oxide synthase isoforms I and III in rat penile spongium tissues].
作者信息
Shi Jun-Ping, Zhao Yong-Mei, Qi Gang
机构信息
Department of Andrology, Shenyang Andrology Hospital, Shenyang, Liaoning 110032, China.
出版信息
Zhonghua Nan Ke Xue. 2003 Dec;9(9):663-5, 668.
OBJECTIVE
To investigate protein and mRNA expression of nitric oxide synthase (NOS) isoforms I and III in rat penile tissues.
METHODS
Penile tissues from male SD rats were investigated. The protein expression of NOS I and III in rat penis was confirmed by immunoprecipitation and Western blot. Gene expression of both NOS I and III was identified by reverse transcription-polymerase chain reaction.
RESULTS
The mRNA and protein products of NOS I and III are both expressed in normal rat penile spongium tissues. But, NOS III mRNA and protein products are more highly expressed than those of NOS.
CONCLUSIONS
These findings support the possibility that penile erection is regulated by different NOS isoforms in rat penile tissues.
目的
研究一氧化氮合酶（NOS）同工酶I和III在大鼠阴茎组织中的蛋白质和mRNA表达。
方法
对雄性SD大鼠的阴茎组织进行研究。通过免疫沉淀和蛋白质印迹法证实大鼠阴茎中NOS I和III的蛋白质表达。通过逆转录-聚合酶链反应鉴定NOS I和III的基因表达。
结果
NOS I和III的mRNA和蛋白质产物均在正常大鼠阴茎海绵体组织中表达。但是，NOS III的mRNA和蛋白质产物比NOS的表达更高。
结论
这些发现支持大鼠阴茎组织中不同的NOS同工酶调节阴茎勃起的可能性。
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