Shi Jun-Ping, Zhao Yong-Mei, Song Yu-Tong
Department of Andrology, Shenyang Andrology Hospital, 32, Beiling Street, Shenyang 110032, China.
Asian J Androl. 2003 Jun;5(2):117-20.
To investigate the effect of aging on the expression of nitric oxide synthase I (NOS I) and the activity of NOS in rat penis.
Sixty male rats from 3 age groups (adult, old and senescent) were investigated. The expression of NOS I protein and mRNA in rat penis were detected by Western blot and RT-PCR respectively and the NOS activity, with ultraviolet spectrophotometry.
In the old and senescent group, NOS I protein expression was significantly decreased as compared with the adult. NOS I mRNA expression was well correlated with the protein expression. NOS activity was not statistically different between the adult and old groups, but it was significantly reduced in the senescent compared with the adult group (P<0.01).
The aging-induced decreases in NOS I expression and NOS activity may be one of the main mechanisms leading to erectile dysfunction in the senescent rats.
研究衰老对大鼠阴茎中一氧化氮合酶I（NOS I）表达及NOS活性的影响。
对60只来自3个年龄组（成年、老年和衰老）的雄性大鼠进行研究。分别采用蛋白质免疫印迹法和逆转录聚合酶链反应（RT-PCR）检测大鼠阴茎中NOS I蛋白和mRNA的表达，并用紫外分光光度法检测NOS活性。
与成年组相比，老年组和衰老组中NOS I蛋白表达显著降低。NOS I mRNA表达与蛋白表达高度相关。成年组和老年组之间NOS活性无统计学差异，但与成年组相比，衰老组NOS活性显著降低（P<0.01）。
衰老导致的NOS I表达和NOS活性降低可能是老年大鼠勃起功能障碍的主要机制之一。
