Sinski Joseph F, Compton Bradley S, Perkins B Shea, Nicoson Michael C
Bellarmine University, Department of Chemistry, 2001 Newberg Road, Louisville, Kentucky 40205, USA.
Appl Spectrosc. 2004 Jan;58(1):91-5. doi: 10.1366/000370204322729513.
Samples of Mycobacterium PRY-1 inoculated motor oil are subjected to three-dimensional fluorescence spectroscopy to document the shifting of excitation/emission maxima as the solutions undergo serial dilutions. Effects such as self-quenching of individual polycyclic aromatic hydrocarbons (PAHs) and energy transfer between PAHs combine to produce large red-shifts in the resulting fluorescence emission spectra. This process is repeated over a series of weeks and is compared to preceding spectra to gauge the microbial degradation of the petroleum. Results show a two-fold, or 75%, PAH contaminant degradation by Mycobacterium PRY-1 over a 140-day growth period.
将接种了分枝杆菌PRY-1的机油样本进行三维荧光光谱分析，以记录溶液进行系列稀释时激发/发射最大值的变化。单个多环芳烃（PAH）的自猝灭以及PAH之间的能量转移等效应共同作用，导致所得荧光发射光谱出现大幅红移。这个过程在一系列的几周内重复进行，并与之前的光谱进行比较，以评估石油的微生物降解情况。结果表明，在140天的生长周期内，分枝杆菌PRY-1使PAH污染物降解了两倍，即75%。
