Landgraf S
Institute of Physical and Theoretical Chemistry, Graz University of Technology, Technikerstrasse 4/I, A-8010 Graz, Austria.
J Biochem Biophys Methods. 2004 Oct 29;61(1-2):125-34. doi: 10.1016/j.jbbm.2004.04.003.
Ultrabright light emitting diodes (LEDs) are an inexpensive alternative to laser diodes (LDs) and other short wavelength emitting light sources. They have a high stability, a long lifetime, and a very low power consumption. A large number of publications are already available for fluorescence applications using this type of LEDs. Most of them are describing fluorescence intensity measurements. Only some of them are dealing with time-resolved methods, like single photon timing. LED modulation fluorometry is a very recent application, which can also be used for environmental investigations, like the detection of polycyclic aromatic hydrocarbons (PAHs). This article demonstrates the possible application of ultrabright LEDs for the time-resolved fluorescence detection of crude oil contaminated samples.
超亮发光二极管（LED）是激光二极管（LD）和其他短波长发光光源的廉价替代品。它们具有高稳定性、长寿命和极低的功耗。关于使用此类LED的荧光应用，已有大量出版物。其中大多数描述的是荧光强度测量。只有少数涉及时间分辨方法，如单光子计时。LED调制荧光法是一种非常新的应用，也可用于环境调查，如多环芳烃（PAH）的检测。本文展示了超亮LED在原油污染样品的时间分辨荧光检测中的可能应用。
