Buddaraju Ajay Kumar V, Van Dyke Rebecca W
Medical Service, 11R Research Service, Ann Arbor VA Hospital, 2215 Fuller Road, Ann Arbor, Michigan 48105, USA.
Comp Med. 2003 Dec;53(6):616-21.
Animal beddings, such as pine products, and environmental factors are known to induce liver drug-metabolizing cytochrome P450 enzymes. We observed that a change to pine-based rat bedding altered baseline and cAMP-stimulated rates of acidification in rat liver endosomes, apparently by decreasing ATP-dependent proton transport in the presence and absence of chloride. Although cAMP altered phosphorylation of protein kinase B and extracellular signal-regulated kinases 1 and 2 (ERK 1,2) and p38 mitogen-activated protein kinases, changes in housing conditions did not affect baseline or cAMP-stimulated values of these or other selected signaling molecules. We conclude that compounds in rat bedding may alter not only drug metabolism, but also aspects of endocytosis.
动物垫料，如松木制品，以及环境因素已知会诱导肝脏药物代谢细胞色素P450酶。我们观察到，更换为以松木为基础的大鼠垫料会改变大鼠肝脏内体的基线酸化率和cAMP刺激的酸化率，这显然是通过在存在和不存在氯离子的情况下降低ATP依赖性质子转运来实现的。尽管cAMP改变了蛋白激酶B和细胞外信号调节激酶1和2（ERK 1,2）以及p38丝裂原活化蛋白激酶的磷酸化，但饲养条件的变化并未影响这些或其他选定信号分子的基线值或cAMP刺激值。我们得出结论，大鼠垫料中的化合物不仅可能改变药物代谢，还可能改变内吞作用的某些方面。
