采用液相色谱-串联质谱法对人血清中的胸腺肽α1进行定量分析。
Quantitative analysis of thymosin alpha1 in human serum by LC-MS/MS.
作者信息
Tuthill C W, Rudolph A, Li Y, Tan B, Fitzgerald T J, Beck S R, Li Y X
机构信息
SciClone Pharmaceuticals, Inc. 901 Mariner's Island Blvd., San Mateo, CA 94404-159, USA.
出版信息
AAPS PharmSciTech. 2000 May 14;1(2):E11. doi: 10.1208/pt010211.
Abstract
A high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed to measure the thymosin alpha 1 (Talpha1) concentration in human serum. Tá1 in human serum was determined by solid phase extraction and reverse phase LC-MS/MS. The high-performance liquid chromatography (HPLC) system interfaced with the MS/MS system with a Turbo Ion spray interface. Positive ion detection and multiple reaction monitoring (MRM) mode were used for this human serum quantitation. Eight different concentration standards were used to establish the detection range. Six quality control (QC) and 2 matrix blanks were checked by calibration curves performed on the same day. The lower quantitation limit was 0.5 ng/mL Talpha1 in human serum. Calibration curves were established between 0.5 to 100 ng/mL by weighted linear regression. The correlation coefficients for different days were 0.9955 or greater. Quantitation of Talpha1 by the LC-MS/MS method is fast, accurate, and precise.
摘要
建立了一种高效液相色谱-串联质谱（LC-MS/MS）法来测定人血清中胸腺肽α1（Tα1）的浓度。通过固相萃取和反相LC-MS/MS测定人血清中的Tα1。高效液相色谱（HPLC）系统通过Turbo离子喷雾接口与MS/MS系统相连。采用正离子检测和多反应监测（MRM）模式对人血清进行定量分析。使用八种不同浓度的标准品来确定检测范围。通过同一天绘制的校准曲线对六个质量控制（QC）样品和两个基质空白进行检测。人血清中Tα1的定量下限为0.5 ng/mL。通过加权线性回归在0.5至100 ng/mL之间建立校准曲线。不同日期的相关系数均大于或等于0.9955。采用LC-MS/MS法对Tα1进行定量分析快速、准确且精密。
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