Deng Yuhang, Zhao Haijian, Liu Qingxiang, Zhou Weiyan, Zhang Chuanbao
National Center for Clinical Laboratories, Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, PR China.
National Center for Clinical Laboratories, Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, PR China.
J Mass Spectrom Adv Clin Lab. 2021 Aug 3;21:1-9. doi: 10.1016/j.jmsacl.2021.07.001. eCollection 2021 Aug.
Human C-peptide is secreted in equimolar amounts with insulin by pancreatic beta-cells. Measurement of C-peptide plays an important role in the diagnosis and treatment of diabetes where it is used to evaluate the function of islet cells. However, C-peptide measurement results across different laboratories vary considerably and there is an urgent need to improve comparability between laboratories. As it is sensitive and specific, isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) has made a major contribution and will continue to play a significant role in the standardization of C-peptide measurement. Here, we reviewed the application of ID-LC-MS/MS in C-peptide measurement by discussing the biochemical properties of C-peptide, common sample preparation procedures, and the sensitivity problems often encountered with ID-LC-MS/MS C-peptide measurement. Collectively, these factors are crucial for the development of ID-LC-MS/MS methods for C-peptide measurement. We also discussed the advantages, disadvantages, and progress of implementing ID-LC-MS/MS as a routine measurement tool for C-peptide in clinical laboratories. Finally, we summarized the existing reference system and the status of C-peptide measurement in clinical laboratories to convey the necessity of improving the comparability of C-peptide measurement in clinical laboratories using ID-LC-MS/MS.
人C肽由胰腺β细胞与胰岛素等摩尔分泌。C肽的测定在糖尿病的诊断和治疗中起着重要作用，用于评估胰岛细胞的功能。然而，不同实验室的C肽测量结果差异很大，迫切需要提高实验室之间的可比性。由于同位素稀释液相色谱串联质谱法（ID-LC-MS/MS）灵敏且特异，它已经做出了重大贡献，并将继续在C肽测量的标准化中发挥重要作用。在此，我们通过讨论C肽的生化特性、常见的样品制备程序以及ID-LC-MS/MS C肽测量中经常遇到的灵敏度问题，综述了ID-LC-MS/MS在C肽测量中的应用。总体而言，这些因素对于开发用于C肽测量的ID-LC-MS/MS方法至关重要。我们还讨论了将ID-LC-MS/MS作为临床实验室C肽常规测量工具的优缺点和进展。最后，我们总结了现有的参考系统以及临床实验室中C肽测量的现状，以说明使用ID-LC-MS/MS提高临床实验室中C肽测量可比性的必要性。
