血小板活化因子对小鼠体外受精不同阶段的影响。
The effect of platelet activating factor on different phases of murine in vitro fertilization.
作者信息
Shi H, Miller F, Miller K, Kim M H
机构信息
Department of Obstetrics and Gynecology, Ohio State University Hospitals, Columbus 43210.
出版信息
J Assist Reprod Genet. 1992 Aug;9(4):373-7. doi: 10.1007/BF01203962.
PURPOSE
Our purpose was to examine the effect of platelet activating factor (PAF) in different phases of mouse in vitro fertilization and optimal parameters that would enhance the fertilization rate.
DESIGN AND SETTINGS
Various PAF concentrations (10(-7) to 10(-5) M) were selected to investigate its effect on three phases of mouse in vitro fertilization (i.e., sperm capacitation, sperm/oocyte coincubation, and preimplantation embryo growth) in three experimental groups: (I) with PAF treatment in the first phase, (II) with PAF treatment adopted in the first and second phases, and (III) with PAF treatment implemented in all three phases.
RESULTS
The improvement of the fertilization rate in PAF treatment groups over the control group ranges from 6.5 to 19.0% (P < 0.05-P < 0.001). The highest enhancement of fertilization rate was achieved under the condition of PAF treatment (10(-6) M) through sperm capacitation and sperm/oocyte coincubation phases.
CONCLUSION
The PAF concentration of 10(-6) M in sperm capacitation and sperm/oocyte coincubation yielded the greatest improvement in fertilization. However, continuing PAF treatment after sperm/oocyte coincubation had no beneficial effect on fertilization and preimplantation development.
目的
我们的目的是研究血小板活化因子（PAF）在小鼠体外受精不同阶段的作用以及能提高受精率的最佳参数。
设计与设置
选择不同浓度的PAF（10⁻⁷至10⁻⁵M），在三个实验组中研究其对小鼠体外受精三个阶段（即精子获能、精子/卵子共孵育和植入前胚胎生长）的影响：（I）在第一阶段进行PAF处理；（II）在第一和第二阶段采用PAF处理；（III）在所有三个阶段都进行PAF处理。
结果
PAF处理组的受精率比对照组提高了6.5%至19.0%（P<0.05 - P<0.001）。在精子获能和精子/卵子共孵育阶段采用PAF（10⁻⁶M）处理的条件下，受精率提高最为显著。
结论
在精子获能和精子/卵子共孵育阶段，10⁻⁶M的PAF浓度对受精的改善效果最佳。然而，在精子/卵子共孵育后继续进行PAF处理对受精和植入前发育没有益处。
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