Kaul Zeenia, Yaguchi Tomoko, Kaul Sunil C, Hirano Takashi, Wadhwa Renu, Taira Kazunari
National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 3058562, Japan.
Cell Res. 2003 Dec;13(6):503-7. doi: 10.1038/sj.cr.7290194.
Quantum dots are the nanoparticles that are recently emerging as an alternative to organic fluorescence probes in cell biology and biomedicine, and have several predictive advantages. These include their i) broad absorption spectra allowing visualization with single light source, ii) exceptional photo-stability allowing long term studies and iii) narrow and symmetrical emission spectrum that is controlled by their size and material composition. These unique properties allow simultaneous excitation of different size of quantum dots with a single excitation light source, their simultaneous resolution and visualization as different colors. At present there are only a few studies that have tested quantum dots in cellular imaging. We describe here the use of quantum dots in mortalin imaging of normal and cancer cells. Mortalin staining pattern with quantum dots in both normal and cancer cells mimicked those obtained with organic florescence probes and were considerably stable.
量子点是近年来在细胞生物学和生物医学领域中作为有机荧光探针替代品而新兴的纳米颗粒，具有若干预测优势。这些优势包括：i）宽吸收光谱，可通过单一光源进行可视化；ii）出色的光稳定性，可进行长期研究；iii）窄且对称的发射光谱，其由尺寸和材料组成控制。这些独特性质使得能够用单一激发光源同时激发不同尺寸的量子点，并将它们同时分辨为不同颜色进行可视化。目前仅有少数研究在细胞成像中测试过量子点。我们在此描述量子点在正常细胞和癌细胞的mortalin成像中的应用。正常细胞和癌细胞中量子点的mortalin染色模式与用有机荧光探针获得的模式相似，并且相当稳定。
