[乙型肝炎病毒母婴垂直传播与人类白细胞抗原DR基因区域关联性研究]

[Study on the association between maternal-infantile vertical transmission of hepatitis B virus and human leukocyte antigen DR gene domain].

作者信息

Liu Hai-ying, Kong Bei-hua, Luo Xia, Xu Yong-ping, Dai Min-sheng, Jiang Sen

机构信息

Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2003 Oct;38(10):599-603.

PMID:14728861

Abstract

OBJECTIVE

To study on the association between maternal-infantile vertical transmission of hepatitis B virus and human leukocyte antigen (HLA)-DR gene domain.

METHODS

HLA-DR3, HLA-DR4, HLA-DR13, HLA-DR15 were detected by the technique of polymerase chain reaction-sequence specific primers (PCR-SSP) in maternal and neonatal blood of study group (78 cases) and control group (40 cases).

RESULTS

(1) In the pregnant women of study group, the gene frequency of HLA-DR3 was significantly higher than that in control group (P < 0.05), but the frequency of HLA-DR13 was significantly lower than those in control group (P < 0.05), while the frequencies of HLA-DR4 and HLA-DR15 had no significant difference between the two groups (P > 0.05). (2) The pregnant women with high replication condition of HBV had significantly higher frequency of HLA-DR3 than those with low replication of HBV (P < 0.05). There was no significant difference of other phenotype between the two conditions. (3) The frequency of HLA-DR3 of pregnant women in intrauterine infection group was significantly higher than that of intrauterine non-infection group (P < 0.05). There had no significant difference of the frequencies of HLA-DR4, HLA-DR13, HLA-DR15 between the two groups (P > 0.05). The gene frequencies of HLA-DR3, HLA-DR4, HLA-DR13, HLA-DR15 were found no significant difference between the neonates of the study group and the control group (P > 0.05). Although the gene frequency of HLA-DR3 (30.0%) in neonates of intrauterine infection group was higher than that of intrauterine non-infection group (7.4%), but no statistical difference was found between the two group (P > 0.05).

CONCLUSIONS

HLA-DR3 is positively correlated with chronic HBV carrying and high replication conditions of pregnant women. HLA-DR13 correlated negatively with chronic HBV carrying of the pregnant women. HLA-DR3 carriers of pregnant women and their fetuses have a susceptibility of HBV infection.

摘要

目的

研究乙型肝炎病毒母婴垂直传播与人类白细胞抗原（HLA）-DR基因区域之间的关联。

方法

采用聚合酶链反应-序列特异性引物（PCR-SSP）技术检测研究组（78例）和对照组（40例）孕妇及新生儿血液中的HLA-DR3、HLA-DR4、HLA-DR13、HLA-DR15。

结果

（1）研究组孕妇中，HLA-DR3基因频率显著高于对照组（P<0.05），但HLA-DR13频率显著低于对照组（P<0.05），而HLA-DR4和HLA-DR15频率在两组间无显著差异（P>0.05）。（2）HBV高复制状态的孕妇HLA-DR3频率显著高于低复制状态的孕妇（P<0.05）。两种状态下其他表型无显著差异。（3）宫内感染组孕妇HLA-DR3频率显著高于宫内未感染组（P<0.05）。两组间HLA-DR4、HLA-DR13、HLA-DR15频率无显著差异（P>0.05）。研究组新生儿与对照组新生儿的HLA-DR3、HLA-DR4、HLA-DR13、HLA-DR15基因频率无显著差异（P>0.05）。虽然宫内感染组新生儿HLA-DR3基因频率（30.0%）高于宫内未感染组（7.4%），但两组间无统计学差异（P>0.05）。