Koster Maranke I, Kim Soeun, Mills Alea A, DeMayo Francesco J, Roop Dennis R
Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Genes Dev. 2004 Jan 15;18(2):126-31. doi: 10.1101/gad.1165104. Epub 2004 Jan 16.
Development of stratified epithelia, such as the epidermis, requires p63 expression. The p63 gene encodes isoforms that contain (TA) or lack (DeltaN) a transactivation domain. We demonstrate that TAp63 isoforms are the first to be expressed during embryogenesis and are required for initiation of epithelial stratification. In addition, TAp63 isoforms inhibit terminal differentiation, suggesting that TAp63 isoforms must be counterbalanced by DeltaNp63 isoforms to allow cells to respond to signals required for maturation of embryonic epidermis. Our data demonstrate that p63 plays a dual role: initiating epithelial stratification during development and maintaining proliferative potential of basal keratinocytes in mature epidermis.
分层上皮组织（如表皮）的发育需要p63的表达。p63基因编码含有（TA）或缺乏（DeltaN）反式激活结构域的异构体。我们证明TAp63异构体是胚胎发育过程中首先表达的，并且是上皮分层起始所必需的。此外，TAp63异构体抑制终末分化，这表明TAp63异构体必须被DeltaNp63异构体所平衡，以使细胞能够响应胚胎表皮成熟所需的信号。我们的数据表明p63发挥双重作用：在发育过程中启动上皮分层，并维持成熟表皮中基底角质形成细胞的增殖潜能。
