Wierup N, Kuhar M, Nilsson B O, Mulder H, Ekblad E, Sundler F
Department of Physiological Sciences, Section of Neuroendocrine Cell Biology, Lund University, Lund, Sweden.
J Histochem Cytochem. 2004 Feb;52(2):169-77. doi: 10.1177/002215540405200204.
Cocaine- and amphetamine-regulated transcript (CART) is an anorexigenic peptide widely expressed in the central and peripheral, including the enteric, nervous systems. CART is also expressed in pituitary endocrine cells, adrenomedullary cells, islet somatostatin cells, and in rat antral gastrin cells. We used immunocytochemistry (IHC) and in situ hybridization (ISH) to study CART expression in developing rat pancreas. We also examined co-expression of CART and islet hormones and developmental markers and the effect of CART on proliferation using clonal insulin cells (INS-1 832/13). A major portion of each of the islet cell types, except the ghrelin cells, expressed CART during a period before and around birth. Two weeks postnatally, CART expression was restricted to somatostatin cells. Pre- and early postnatally, many of the CART-expressing cells co-expressed cytokeratin 20 (CK20), a marker of duct cells and islet precursor cells, the trophic hormone gastrin, and a smaller subpopulation also harbored the proliferation marker Ki67. CART was also expressed in pancreatic nerve fibers, both sensory and autonomic, and in ganglion nerve cell bodies. Although highly expressed in the developing islets, CART did not affect proliferation of INS-1 cells. We have demonstrated that CART is expressed in several islet cell types during rat development but is restricted to somatostatin cells and neurons in the adult rat.
可卡因和苯丙胺调节转录肽（CART）是一种厌食肽，广泛表达于中枢和外周，包括肠神经系统。CART也表达于垂体内分泌细胞、肾上腺髓质细胞、胰岛生长抑素细胞以及大鼠胃窦促胃液素细胞中。我们采用免疫细胞化学（IHC）和原位杂交（ISH）技术研究CART在发育中的大鼠胰腺中的表达情况。我们还检测了CART与胰岛激素及发育标志物的共表达情况，以及利用克隆胰岛素细胞（INS-1 832/13）研究CART对细胞增殖的影响。除胃饥饿素细胞外，每种胰岛细胞类型的大部分在出生前及出生前后一段时间均表达CART。出生后两周，CART表达局限于生长抑素细胞。在出生前及出生后早期，许多表达CART的细胞共表达细胞角蛋白20（CK20），这是导管细胞和胰岛前体细胞的标志物、营养激素促胃液素，还有一小部分细胞群同时含有增殖标志物Ki67。CART也表达于胰腺感觉和自主神经纤维以及神经节神经细胞体中。尽管CART在发育中的胰岛中高表达，但它并不影响INS-1细胞的增殖。我们已经证明，CART在大鼠发育过程中表达于几种胰岛细胞类型中，但在成年大鼠中局限于生长抑素细胞和神经元。
