用于异常毕赤酵母临床和非临床分离株菌株分型的ITS和IGS1区域比较

Comparison of ITS and IGS1 regions for strain typing of clinical and non-clinical isolates of Pichia anomala.

作者信息

Sutar Rajeshwari, David Joseph K, Ganesan K, Ghosh Anup K, Singhi Sunit, Chakrabarti Arunaloke, Bachhawat Anand K

机构信息

Institute of Microbial Technology, Sector 39-A, Chandigarh, India 160 036 2Postgraduate Institute of Medical Education and Research, Chandigarh, India 160 012.

出版信息

J Med Microbiol. 2004 Feb;53(Pt 2):119-123. doi: 10.1099/jmm.0.05436-0.

DOI:10.1099/jmm.0.05436-0

PMID:14729932

Abstract

Pichia anomala is an emerging nosocomial pathogen and there is a need for methods that distinguish between different P. anomala strains. In the typing of several clinical as well as non-clinical P. anomala strains, the sequence variation of the internal transcribed spacer (ITS) was found to be inadequate for typing purposes. The intergenic spacer 1 (IGS1) region of the rDNA of several P. anomala strains was therefore investigated in detail. The IGS1 region (which varied from 1213 to 1231 bp in length) was interspersed with repeats and had more variation than the ITS regions. Comparative analysis in cases where analysis by the ITS was ambiguous clearly revealed the IGS1 region to be a more discriminatory tool in the typing of P. anomala strains.

摘要

异常毕赤酵母是一种新出现的医院病原体，因此需要有区分不同异常毕赤酵母菌株的方法。在对多个临床及非临床异常毕赤酵母菌株进行分型时，发现内部转录间隔区（ITS）的序列变异不足以用于分型。因此，对多个异常毕赤酵母菌株的核糖体DNA的基因间隔区1（IGS1）区域进行了详细研究。IGS1区域（长度在1213至1231 bp之间变化）散布着重复序列，并且比ITS区域具有更多变异。在ITS分析结果不明确的情况下进行的比较分析清楚地表明，IGS1区域在异常毕赤酵母菌株分型中是一种更具区分性的工具。