An Chung-Il, Sawada Aki, Fukusaki Ei-ichiro, Kobayashi Akio
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan.
Biosci Biotechnol Biochem. 2003 Dec;67(12):2674-7. doi: 10.1271/bbb.67.2674.
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.
双链RNA（dsRNA）通过一种称为RNA干扰（RNAi）的过程在真核生物中诱导序列特异性基因沉默。RNAi现在被用作许多真核生物（包括植物）功能基因组学的强大工具。我们在此报告了一种使用拟南芥叶肉细胞原生质体和悬浮培养细胞（细胞系T87）的dsRNA介导的瞬时RNAi检测系统。将dsRNA导入原生质体导致目标转基因的显著沉默。我们的检测系统将为在模式植物拟南芥的原生质体中诱导RNAi提供一种方便且高效的方法。
