An Chung-Il, Sawada Aki, Kawaguchi Yoshiaki, Fukusaki Ei-ichiro, Kobayashi Akio
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan.
Biosci Biotechnol Biochem. 2005 Feb;69(2):415-8. doi: 10.1271/bbb.69.415.
RNA interference is a powerful technique for suppressing gene functions in many eukaryotes including plants. Here we show that introduction of double-stranded RNA into Arabidopsis protoplasts leads to marked silencing of endogenous genes, as observed previously for transgenes [Biosci. Biotechnol. Biochem., 67, 2674-2677 (2003)]. This simple system should be useful for functional analysis of genes involved in fundamental cellular processes.
RNA干扰是在包括植物在内的许多真核生物中抑制基因功能的一项强大技术。我们在此表明，将双链RNA导入拟南芥原生质体可导致内源基因显著沉默，这与之前在转基因中观察到的情况相同[《生物科学、生物技术与生物化学》，67，2674 - 2677(2003)]。这个简单的系统对于参与基本细胞过程的基因的功能分析应该是有用的。
