Sun Ying-jie, Lü Peng, Ling Jian-ya, Zhang Han-xing, Chen Chang, Zhang Chang-kai
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
Yao Xue Xue Bao. 2003 Sep;38(9):690-4.
To rapidly separate and determine the nucleosides from natural and cultured Cordyceps kyushuensis Kob., and to compare the content of cordycepin and adenosine in different parts of Cordyceps kyushuensis Kob., which are the main nucleoside active components in medicinal fungus belonging to Cordyceps (Fr.) Link.
The nucleosides were separated and determined by the high performance capillary zone electrophoresis (CZE). Beckman P/ACE system MDQ apparatus equipped with a PDA detector and a uncoated fused-silica capillary (41 cm x 45 microns ID, 30 cm effective length) were used. The experimental conditions were as follows: the running buffer was borax solution (adjust to pH 9.4 with sodium hydroxide), applied voltage was 20 kV, operated temperature was 20 degrees C and the detector wavelength was 258 nm. The content of cordycepin and adenosine in the fruiting body, stroma and host worm of natural and cultured C. kyushuensis were respectively investigated and quantitatively analyzed.
There are at least 8 kinds of nucleoside or nitrogen base in Cordyceps kyushuensis Kob. The content of cordycepin which is a bio-active substance with anti-tumor activity in C. kyushuensis is significantly higher than that in C. sinensis and C. militaris, and furthermore the cordycepin in the cultured C. kyushuensis is notably higher than the natural one. Adenosine was mainly found from the stroma of C. kyushuensis, While the cordycepin content is high in the stroma of both natural and cultured C. kyushuensis as well as in the host worm of the cultured one.
There are some differences about the nucleoside components between the natural and cultured C. kyushuensis and between the different parts of them. With a high cordycepin content, C. kyushuensis should have a considerable medicinal potential.
快速分离并测定天然及培养的九州虫草中的核苷类成分，并比较九州虫草不同部位（其为虫草属药用真菌中的主要核苷活性成分）中虫草素和腺苷的含量。
采用高效毛细管区带电泳（CZE）分离并测定核苷类成分。使用配备有光电二极管阵列检测器（PDA）及未涂层熔融石英毛细管（41 cm×45μm内径，有效长度30 cm）的贝克曼P/ACE系统MDQ仪器。实验条件如下：运行缓冲液为硼砂溶液（用氢氧化钠调至pH 9.4），施加电压为20 kV，操作温度为20℃，检测波长为258 nm。分别对天然及培养的九州虫草子实体、菌核和寄主虫体中虫草素和腺苷的含量进行研究及定量分析。
九州虫草中至少含有8种核苷或含氮碱基。具有抗肿瘤活性的生物活性物质虫草素在九州虫草中的含量显著高于中华虫草和蛹虫草，而且培养的九州虫草中的虫草素含量明显高于天然的。腺苷主要存在于九州虫草的菌核中，而天然及培养的九州虫草菌核以及培养的九州虫草寄主虫体中虫草素含量均较高。
天然及培养的九州虫草之间及其不同部位的核苷类成分存在一些差异。九州虫草虫草素含量高，具有相当大的药用潜力。
