Gong Ben-Jiao, Rong Rong, Zhang Guo-Ying, Ling Jian-Ya, Zhang Chang-Kai
State Key Laboratory of Microbial Technology, Shandong University, Ji'nan 250100, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2011 Sep;31(9):2420-3.
Fourier-transform infrared spectroscopy (FTIR) and second derivative spectra were used to analyze and evaluate the different parts of Cordyceps kyushuensis Kob in the present work. The results showed that C. kyushuensis contained proteins, polysaccharides, nucleosides, lipids and other active ingredients, the single-dimensional IR spectra of the various parts were highly similar, the similarity coefficient between the cultured stroma and medium reached up to 0.992 7, and the natural stroma was more different from the parasites, with similarity coefficient of 0.949 9. Second derivative spectrum further enriched and confirmed the feature of corresponding spectrum peaks, proved the existence of active substances such as cordycepin and adenosine, and prompted the presence of alpha- and beta-glycosidic bonds. The diversity and complexity of chemical constituents in different parts of C. kyushuensis were synthetically described by IR spectra, which provided a fast, comprehensive and objective approach to the analysis and evaluation of the imperceptible differences, and the quality control of Cordyceps. This work supplies a theoretical basis for development and utilization of genetic resources C. kyushuensis.
本研究采用傅里叶变换红外光谱（FTIR）和二阶导数光谱对九州虫草的不同部位进行分析和评价。结果表明，九州虫草含有蛋白质、多糖、核苷、脂质等活性成分，各部位的一维红外光谱高度相似，培养基质与培养基之间的相似系数高达0.992 7，天然基质与虫体差异较大，相似系数为0.949 9。二阶导数光谱进一步丰富和确认了相应光谱峰的特征，证明了虫草素和腺苷等活性物质的存在，并提示了α-和β-糖苷键的存在。红外光谱综合描述了九州虫草不同部位化学成分的多样性和复杂性，为分析和评价其细微差异及虫草质量控制提供了一种快速、全面、客观的方法。本研究为九州虫草遗传资源的开发利用提供了理论依据。
