一种用于确定DNA结合选择性和亲和力的荧光嵌入剂置换分析法。
A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity.
作者信息
Tse Winston C, Boger Dale L
机构信息
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
出版信息
Acc Chem Res. 2004 Jan;37(1):61-9. doi: 10.1021/ar030113y.
PMID:14730995
Abstract
A summary of the qualitative and quantitative elements of a fluorescent intercalator displacement (FID) assay useful for establishing the DNA binding selectivity, affinity, stoichiometry, and binding site size and distinguishing modes of DNA binding is provided.
摘要
本文提供了一种荧光嵌入剂置换（FID）分析方法的定性和定量要素总结，该方法可用于确定DNA结合的选择性、亲和力、化学计量、结合位点大小以及区分DNA结合模式。
Zotero 插件