一种用于确定DNA结合选择性和亲和力的荧光嵌入剂置换分析。
A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity.
作者信息
Tse Winston C, Boger Dale L
机构信息
Gilead Sciences, Foster City, California, USA.
出版信息
Curr Protoc Nucleic Acid Chem. 2005 Apr;Chapter 8:Unit 8.5. doi: 10.1002/0471142700.nc0805s20.
PMID:18428943
Abstract
A protocol for a fluorescent intercalator displacement (FID) assay useful for establishing DNA binding selectivity, affinity, stoichiometry, and binding site size, and for distinguishing modes of DNA binding is presented.
摘要
本文介绍了一种荧光嵌入剂置换（FID）分析方法的方案，该方法可用于确定DNA结合选择性、亲和力、化学计量和结合位点大小，以及区分DNA结合模式。
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