de Bruin A H, Parolis H, Parolis L A
School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
Carbohydr Res. 1992 Nov 4;235:199-209. doi: 10.1016/0008-6215(92)80088-i.
The structure of the capsular antigen of E. coli O8:K102:H- was investigated by methylation analysis, beta-elimination of the methylated polysaccharide, lithium-ethylenediamine mediated degradation, and by 1D and 2D 1H and 13C NMR spectroscopy of the lithium-degraded and native polysaccharides. The capsular antigen was shown to have the following branched pentasaccharide repeating unit: [formula: see text]
通过甲基化分析、甲基化多糖的β-消除、锂-乙二胺介导的降解以及对锂降解多糖和天然多糖进行一维和二维氢谱与碳谱核磁共振波谱,对大肠杆菌O8:K102:H-的荚膜抗原结构进行了研究。结果表明,该荚膜抗原具有以下分支五糖重复单元:[分子式:见原文]