一株肠聚集性大肠杆菌的O抗原多糖的结构研究

Structural studies of the O-antigenic polysaccharide of an enteroaggregative Escherichia coli strain.

作者信息

Weintraub A, Leontein K, Widmalm G, Vial P A, Levine M M, Lindberg A A

机构信息

Department of Clinical Bacteriology, Karolinska Institute, Huddinge Hospital, Sweden.

出版信息

Eur J Biochem. 1993 Apr 15;213(2):859-64. doi: 10.1111/j.1432-1033.1993.tb17829.x.

DOI:10.1111/j.1432-1033.1993.tb17829.x

PMID:7682949

Abstract

The polysaccharide part of the lipopolysaccharide obtained from an enteroaggregative Escherichia coli strain isolated from a young child with diarrhoea in Santiago, Chile (strain 73-1) was investigated. Sugar and methylation analyses of native and partially degraded polysaccharide together with 1H-NMR and 13C-NMR spectroscopy revealed that the polysaccharide is built of pentasaccharide repeating units. The structure of the repeating unit of E. coli strain 73-1 O-polysaccharide is (formula: see text)

摘要

对从智利圣地亚哥一名患腹泻幼儿分离出的肠聚集性大肠杆菌菌株（菌株73-1）获得的脂多糖的多糖部分进行了研究。对天然和部分降解的多糖进行糖和甲基化分析，并结合1H-NMR和13C-NMR光谱分析表明，该多糖由五糖重复单元构成。大肠杆菌菌株73-1 O-多糖重复单元的结构为（分子式：见正文）