Asai M, Berryere T G, Schmutz S M
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Anim Genet. 2004 Feb;35(1):34-9. doi: 10.1111/j.1365-2052.2003.01079.x.
Polled, or the absence of horns, is a desirable trait for many cattle breeders. However, the presence of scurs, which are small horn-like structures that are not attached to the skull, can lower the value of an animal. The scurs trait has been reported as sex influenced. Using a genome scan with 162 autosomal microsatellite markers genotyped across three full-sib families, the scurs locus was mapped near BMS2142 on cattle chromosome 19 (LOD = 4.21). To more precisely map scurs, the families from the initial analysis and three additional families were genotyped for 16 microsatellite markers and SNPs in three genes on chromosome 19. In this subsequent analysis, the scurs locus was mapped 4 cM distal of BMS2142 (LOD = 4.46) and 6 cM proximal to IDVGA46 (LOD = 2.56). ALOX12 and MFAP4 were the closest genes proximal and distal, respectively, to the scurs locus. Three microsatellite markers on the X chromosome were genotyped across these six families but were not linked to scurs, further demonstrating that this trait was not sex linked. Because the polled locus has been mapped to the centromeric end of chromosome 1 and scurs has now been mapped to chromosome 19, these two traits are not linked in Bos taurus.
无角,即没有牛角,对许多养牛者来说是一个理想的性状。然而,有小角状结构且不与头骨相连的小角的存在,会降低动物的价值。小角性状已被报道为受性别影响。通过对三个全同胞家系的162个常染色体微卫星标记进行基因分型的基因组扫描,将小角基因座定位在牛19号染色体上靠近BMS2142的位置(LOD = 4.21)。为了更精确地定位小角基因座,对初始分析中的家系以及另外三个家系进行了19号染色体上三个基因中的16个微卫星标记和单核苷酸多态性的基因分型。在后续分析中,小角基因座被定位在BMS2142远端4厘摩处(LOD = 4.46)和IDVGA46近端6厘摩处(LOD = 2.56)。ALOX12和MFAP4分别是最靠近小角基因座近端和远端的基因。对这六个家系的X染色体上的三个微卫星标记进行了基因分型,但它们与小角不连锁,进一步证明该性状不是性连锁的。由于无角基因座已被定位到1号染色体的着丝粒末端,而小角现在已被定位到19号染色体,所以这两个性状在普通牛中不连锁。