Liang Y R
Department of Pathology, Hunan Medical University, Changsha.
Zhonghua Bing Li Xue Za Zhi. 1992 Aug;21(4):212-4.
HLA-DR immunohistochemistry of 110 liver biopsies including 85 hepatitis (in 7 of them immunoelectron microscopy was done), 15 other liver diseases and 10 normal livers were studied. In the hepatitis group, Kupffer cells, sinusoidal walls, inflammatory cells and necrotic areas were strongly positive for HLA-DR; whereas in other liver diseases, they were only weakly positive. In hepatitis, CAH showed the strongest staining and a "reticular pattern". Expression of HLA-DR on hepatocyte membrane was only found in a few hepatocytes. In 5 cases of double labelling, no significant relation between HLA-DR and HBcAg was found. Immune EM identified both membrane and cytoplasm of Kupffer cells to be HLA-DR positive. Hepatocytes in focal necrosis and submassive necrosis were positive, especially in the endoplasmic reticulum. Between some hepatocytes, consecutive positive lines were found indicating that the membrane of hepatocyte could express HLA-DR.
对110例肝活检组织进行了HLA - DR免疫组化研究，其中包括85例肝炎患者（7例进行了免疫电镜检查）、15例其他肝脏疾病患者以及10例正常肝脏组织。在肝炎组中，库普弗细胞、肝血窦壁、炎症细胞及坏死区域HLA - DR呈强阳性；而在其他肝脏疾病中，它们仅呈弱阳性。在肝炎中，慢性活动性肝炎染色最强，呈“网状模式”。仅在少数肝细胞中发现肝细胞膜上有HLA - DR表达。在5例双重标记病例中，未发现HLA - DR与乙肝核心抗原之间有显著相关性。免疫电镜显示库普弗细胞的细胞膜和细胞质均为HLA - DR阳性。局灶性坏死和亚大块坏死的肝细胞呈阳性，尤其是在内质网。在一些肝细胞之间发现连续的阳性线条，表明肝细胞膜可表达HLA - DR。
