Feng Jian-Xun, Ji Sheng-Jian, Shi Yong-Hui, Xu Yu, Wei Gang, Zhu Yu-Xian
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
Acta Biochim Biophys Sin (Shanghai). 2004 Jan;36(1):51-6. doi: 10.1093/abbs/36.1.51.
Using the suppression subtractive hybridization method, we isolated five gene families, including proline-rich proteins (PRPs), arabinogalactan proteins (AGPs), expansins, tubulins and lipid transfer proteins (LTPs), from fast elongating cotton fiber cells. Expression profile analysis using cDNA array technology showed that most of these gene families were highly expressed during early cotton fiber developmental stages (0 C20 days post anthesis, DPA). Many transcripts accumulated over 50-fold in 10 DPA fiber cells than in 0 DPA samples. The entire gene family --AGP, together with 20 individual members in other 4 gene families, are reported in cotton for the first time. Accumulation of cell wall proteins, wall loosening enzymes, microtubules and lipid transfer protein may contribute directly to the elongation and development of fiber cells.
利用抑制性消减杂交方法，我们从快速伸长的棉纤维细胞中分离出了五个基因家族，包括富含脯氨酸的蛋白质（PRP）、阿拉伯半乳聚糖蛋白（AGP）、伸展蛋白、微管蛋白和脂类转移蛋白（LTP）。使用cDNA阵列技术进行的表达谱分析表明，这些基因家族中的大多数在棉花纤维发育早期阶段（开花后0至20天，DPA）高度表达。许多转录本在10 DPA纤维细胞中的积累量比在0 DPA样本中高出50倍以上。整个AGP基因家族以及其他4个基因家族中的20个个体成员，首次在棉花中被报道。细胞壁蛋白、细胞壁松弛酶、微管和脂类转移蛋白的积累可能直接有助于纤维细胞的伸长和发育。
