Samuel Michael D, Shadduck Daniel J, Goldberg Diana R, Wilson Mark A, Joly Damien O, Lehr Margaret A
U.S. Geological Survey, National Wildlife Health Center, 6006 Schroeder Road, Madison, Wisconsin 53711, USA.
J Wildl Dis. 2003 Oct;39(4):798-807. doi: 10.7589/0090-3558-39.4.798.
We cultured 126 Pasteurella multocida isolates, 92 from water and 34 from sediment samples collected from wetlands in the Pacific and Central flyways of the United States between 1996 and 1999. Most (121) of the isolates were P. multocida serotype 1, but serotypes 3, 3/4, 10, and 11 were also found. Many (82) of the isolates were further characterized by DNA fingerprinting procedures and tested in Pekin ducks for virulence. Almost all the serotype 1 isolates we tested caused mortality in Pekin ducks. Serotype 1 isolates varied in virulence, but the most consistent pattern was higher mortality in male ducks than in females. We found no evidence that isolates found in sediment vs. water, between Pacific and Central flyways, or during El Niño years had consistently different virulence. We also found a number of non-serotype 1 isolates that were avirulent in Pekin ducks. Isolates had DNA fingerprint profiles similar to those found in birds that died during avian cholera outbreaks.
我们培养了126株多杀性巴氏杆菌分离株，其中92株来自于1996年至1999年间从美国太平洋和中部飞行路线的湿地采集的水样，34株来自沉积物样本。大多数（121株）分离株为多杀性巴氏杆菌1型，但也发现了3型、3/4型、10型和11型。许多（82株）分离株通过DNA指纹图谱程序进一步鉴定，并在 Pekin 鸭中进行毒力测试。我们测试的几乎所有1型分离株都导致了 Pekin 鸭死亡。1型分离株的毒力各不相同，但最一致的模式是雄鸭的死亡率高于雌鸭。我们没有发现证据表明在沉积物与水样中发现的分离株、在太平洋和中部飞行路线之间发现的分离株或在厄尔尼诺年期间发现的分离株在毒力上存在持续差异。我们还发现了一些在 Pekin 鸭中无毒力的非1型分离株。分离株的DNA指纹图谱与在禽霍乱疫情期间死亡的鸟类中发现的图谱相似。
