Olsen S C, Holland S D
Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2300 Dayton Ave., Ames, Iowa 50010, USA.
J Wildl Dis. 2003 Oct;39(4):824-9. doi: 10.7589/0090-3558-39.4.824.
From December 1998 through February 1999, a study was conducted in a Brucella-infected bison herd to evaluate the safety of booster vaccination of adult bison (Bison bison) with 6 x 10(9) colony forming units (CFU) of Brucella abortus strain RB51 (SRB51) that had previously been vaccinated as yearlings with 1 x 10(10) CFU of SRB51. Abortions or other adverse effects were not observed after SRB51 booster vaccination. At 10 wk after adult vaccination, pregnant and nonpregnant bison (n = 65) were randomly selected for bacteriologic sampling of targeted maternal tissues during abattoir processing. Fetal tissues were also sampled in pregnant bison. The SR351 recovered from tissue samples of eight of 48 pregnant bison and none of 17 nonpregnant bison. In three of the eight culture-positive bison, SRB51 was recovered from fetal tissues. In three additional bison, one pregnant and two nonpregnant, B. abortus biovar 1 field strain was recovered from internal iliac or supramammary lymphatic tissues. Results of this study suggest the possibility that the SRB51 vaccine can be safely used to booster vaccinate pregnant bison in a Brucella-infected bison herd. Our data also reaffirms the potential for B. abortus field strains to persist in bison until attainment of reproductive age, despite extensive use of vaccination and serologic testing.
1998年12月至1999年2月，在一群感染布鲁氏菌的野牛中进行了一项研究，以评估用6×10⁹菌落形成单位（CFU）的流产布鲁氏菌RB51菌株（SRB51）对成年野牛（美洲野牛）进行加强免疫接种的安全性，这些成年野牛在一岁时曾用1×10¹⁰CFU的SRB51进行过疫苗接种。在进行SRB51加强免疫接种后，未观察到流产或其他不良反应。在成年野牛接种疫苗10周后，在屠宰加工过程中随机选择怀孕和未怀孕的野牛（n = 65）进行靶向母体组织的细菌学采样。还对怀孕野牛的胎儿组织进行了采样。从48头怀孕野牛中的8头以及17头未怀孕野牛中的任何一头的组织样本中均未分离到SRB51。在8头培养阳性的野牛中有3头，从胎儿组织中分离到了SRB51。在另外3头野牛中，1头怀孕和2头未怀孕，从髂内或乳房上淋巴结组织中分离到了流产布鲁氏菌生物变种1野毒株。本研究结果提示，在感染布鲁氏菌的野牛群中，SRB51疫苗可安全用于对怀孕野牛进行加强免疫接种。我们的数据还再次证实，尽管广泛使用疫苗接种和血清学检测，但流产布鲁氏菌野毒株仍有可能在野牛体内持续存在至达到生殖年龄。
