布鲁氏菌病牛种RB51菌株在黑熊中的安全性。

Safety of Brucella abortus strain RB51 in black bears.

作者信息

Olsen Steven C, Rhyan Jack, Gidlewski T, Goff Jesse, Stoffregen W C

机构信息

Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, Iowa 50010, USA.

出版信息

J Wildl Dis. 2004 Jul;40(3):429-33. doi: 10.7589/0090-3558-40.3.429.

DOI:10.7589/0090-3558-40.3.429

PMID:15465709

Abstract

In two studies conducted from October 1999 to March 2000 and December 2000 to April 2001, adult black bears (Ursus americanus) were orally inoculated with 1.4-3.1 x 10(10) colony-forming units (CFU) of Brucella abortus strain RB51 (SRB51, n=12) or 2 ml of 0.15 M NaCl solution (saline, n=11). We did not detect a difference (P>0.05) in antibody titers to SRB51 in serum obtained before vaccination, at 8 wk after vaccination, or at necropsy at 21 or 23 wk after vaccination between SRB51-vaccinated and nonvaccinated bears. The SRB51 vaccine strain was recovered from tissues obtained at necropsy from one of six SRB51-vaccinated bears in study 1, but none of the six SRB51-vaccinated bears in study 2. Vaccination of black bears with SRB51 did not appear to influence (P>0.05) reproductive performance.

摘要

在1999年10月至2000年3月以及2000年12月至2001年4月进行的两项研究中，成年黑熊（美洲黑熊）经口接种了1.4 - 3.1×10¹⁰个布鲁氏菌流产菌株RB51（SRB51，n = 12）的菌落形成单位（CFU），或2毫升0.15 M氯化钠溶液（生理盐水，n = 11）。我们未检测到接种SRB51的黑熊与未接种黑熊在接种前、接种后8周或接种后21或23周尸检时血清中针对SRB51的抗体滴度存在差异（P>0.05）。在研究1中，从6只接种SRB51的黑熊中的1只尸检获得的组织中分离出了SRB51疫苗株，但在研究2的6只接种SRB51的黑熊中均未分离出。用SRB51对黑熊进行疫苗接种似乎并未影响（P>0.05）繁殖性能。