Drogui P, Picher S, Mercier G, Blais J F
Institut National de la Recherche Scientifique (INRS-ETE), Université du Quebec, 2800, rue Einstein, C.P. 7500, Sainte-Foy, Qc, G1V 4C7, Canada.
Environ Technol. 2003 Nov;24(11):1413-23. doi: 10.1080/09593330309385685.
In this study, the results of the leaching of metal sulphide concentrate using organic wastes as culture media for Acidithiobacillus ferrooxidans are summarized. These results indicate that the liquid fraction of municipal sewage sludge, paper mill sludge and pig manure, containing 10% (w v(-1)) pulp density of a pyritic mine waste concentrate can support the growth of the leaching bacteria and allow metal solubilization. The inhibition by dissolved organic carbon (DOC) appeared when the concentration in pig manure liquid fraction and sewage sludge filtrate is higher than 180 mg l(-1) and 500 mg l(-1), respectively. However, increase in organic concentration up to 650 mg l(-1) using paper mill sludge supernatant had no inhibitory effect on the bacterial growth. An important decrease of the DOC has been measured during all bioleaching tests. The organic matter was probably consumed by heterotrophic microorganisms activity. The growth rate of the iron-oxidizing bacteria varied from 0.05 to 0.07 h(-1). The dissolution of pyrite (FeS2) in organic waste media led to a yield of Fe solubilization of about 35%. Copper and zinc were also solubilized during the bioleaching tests. The yields of Cu and Zn solubilization ranged from 12 to 24%.
本研究总结了以有机废弃物作为氧化亚铁硫杆菌的培养基对金属硫化物精矿进行浸出的结果。这些结果表明，城市污水污泥、造纸厂污泥和猪粪的液体部分，在含有10%（w v(-1)）黄铁矿尾矿精矿矿浆密度时，能够支持浸出细菌的生长并实现金属溶解。当猪粪液体部分和污水污泥滤液中溶解有机碳（DOC）的浓度分别高于180 mg l(-1)和500 mg l(-1)时，会出现抑制作用。然而，使用造纸厂污泥上清液将有机浓度提高至650 mg l(-1)时，对细菌生长没有抑制作用。在所有生物浸出试验中均检测到DOC显著降低。有机物可能因异养微生物的活动而被消耗。铁氧化细菌的生长速率在0.05至0.07 h(-1)之间。黄铁矿（FeS2）在有机废弃物培养基中的溶解导致铁溶解率约为35%。在生物浸出试验过程中，铜和锌也被溶解。铜和锌的溶解率在12%至24%之间。
