玉米青贮饲料中伏马毒素B1、B2和B3的液相色谱测定法

Liquid chromatographic determination of fumonisins B1, B2, and B3 in corn silage.

作者信息

Kim Eun-Kyung, Maragos Chris M, Kendra David F

机构信息

Mycotoxin Research Unit, National Center for Agricultural Utilization Research, USDA/ARS, 1815 North University Street, Peoria, Illinois 61604, USA.

出版信息

J Agric Food Chem. 2004 Jan 28;52(2):196-200. doi: 10.1021/jf034934t.

DOI:10.1021/jf034934t

PMID:14733494

Abstract

Corn silage was dried, ground, and then extracted with 0.1 M ethylenediaminetetraacetic acid. The filtrate was applied to a FumoniTest immunoaffinity column. Fumonisins were derivatized with naphthalene-2,3-dicarboxaldehyde, separated on a C(18) liquid chromatographic column, and detected by fluorescence. The detection limits for fumonisin B(1), fumonisin B(2), and fumonisin B(3) were 50, 25, and 25 ng/g of dried silage, respectively. Recoveries of fumonisin B(1), fumonisin B(2), and fumonisin B(3) from wet and dried corn silage spiked over the range of 100-5000 ng/g averaged 91-106%. The method was applied to corn silage samples collected from the midwestern area of the United States during 2001-2002. Of 89 corn silage samples, fumonisin B(1), fumonisin B(2), and fumonisin B(3) were found in 86 (97%), 64 (72%), and 51 (57%) of the samples. The mean positive levels of fumonisin B(1), fumonisin B(2), and fumonisin B(3) were 615, 93, and 51 ng/g, respectively, in dried silage. This suggests that fumonisins may be frequent low level contaminants in corn silage.

摘要

玉米青贮饲料经干燥、研磨后，用0.1 M乙二胺四乙酸进行提取。将滤液应用于伏马菌素检测免疫亲和柱。伏马菌素用萘-2,3-二甲醛衍生化，在C(18)液相色谱柱上分离，并用荧光检测。伏马菌素B(1)、伏马菌素B(2)和伏马菌素B(3)在干燥青贮饲料中的检测限分别为50、25和25 ng/g。在加标量为100 - 5000 ng/g的湿玉米青贮饲料和干玉米青贮饲料中，伏马菌素B(1)、伏马菌素B(2)和伏马菌素B(3)的回收率平均为91% - 106%。该方法应用于2001 - 2002年从美国中西部地区采集的玉米青贮饲料样品。在89个玉米青贮饲料样品中，86个(97%)、64个(72%)和51个(57%)样品中分别检测到伏马菌素B(1)、伏马菌素B(2)和伏马菌素B(3)。干青贮饲料中伏马菌素B(1)、伏马菌素B(2)和伏马菌素B(3)的平均阳性水平分别为615、93和51 ng/g。这表明伏马菌素可能是玉米青贮饲料中常见的低水平污染物。