Brohée D, Higuet N
Department of Internal Medicine, Université Libre de Bruxelles, C.H.U. André Vésale, Montigny-le-Tilleul, Belgium.
Cytobios. 1992;71(285):105-11.
Following phytohaemagglutinin A stimulation, CD45RA positive monocytes increased from 12.2 +/- 8.9% to 63.5 +/- 8.8% (p < 0.001), without a significant change in cell surface antigen density. In contrast, HLA-DQ antigen, also expressed in 76.8 +/- 10.5% of the monocytes after PHA stimulation for 48 h, revealed a marked enhancement in fluorescence intensity (p < 0.001). This up-regulation is already evident in unstimulated cultures. The percentages of CD45RA and HLA-DQ positive monocytes were correlated (r = 0.80, p < 0.001), substantiating a previous clinical observation. CD45RA expression may probe activated mono/macrophages.
在植物血凝素A刺激后，CD45RA阳性单核细胞从12.2±8.9%增加至63.5±8.8%（p<0.001），细胞表面抗原密度无显著变化。相比之下，PHA刺激48小时后，76.8±10.5%的单核细胞也表达的HLA-DQ抗原，其荧光强度显著增强（p<0.001）。这种上调在未刺激的培养物中已经很明显。CD45RA和HLA-DQ阳性单核细胞的百分比具有相关性（r=0.80，p<0.001），证实了先前的临床观察。CD45RA表达可能探测活化的单核/巨噬细胞。
