Marshall M E, Rhoades J L, Mattingly C, Jennings C D
Division of Hematology/Oncology, University of Kentucky Medical Center, Lexington.
Mol Biother. 1991 Dec;3(4):204-6.
Coumarin (1,2-benzopyrone) is a natural substance that appears to have some clinical activity against renal cell carcinoma and malignant melanoma. Preliminary evidence from in vitro and in vivo studies suggests that coumarin possesses immunomodulatory activity. It was reported previously that coumarin therapy resulted in augmented DR antigen expression by peripheral blood monocytes in cancer patients. The purpose of the present study was to examine the effects of coumarin on DR and DQ antigen expression by normal donor peripheral blood mononuclear cells in vitro. Using monoclonal antibody labeling techniques and FACS analysis, it was shown that both DR and DQ antigen expression by peripheral blood mononuclear cells were enhanced over controls after 48 hours of exposure to coumarin. While monocytes normally express these antigens, enhanced expression is consistent with an activated state. These results support the hypothesis that coumarin acts, at least in part, through immune augmentation.
香豆素（1,2 - 苯并吡喃酮）是一种天然物质，似乎对肾细胞癌和恶性黑色素瘤具有一定的临床活性。体外和体内研究的初步证据表明，香豆素具有免疫调节活性。先前有报道称，香豆素治疗可使癌症患者外周血单核细胞的DR抗原表达增加。本研究的目的是在体外检测香豆素对正常供体外周血单个核细胞DR和DQ抗原表达的影响。使用单克隆抗体标记技术和流式细胞术分析表明，暴露于香豆素48小时后，外周血单个核细胞的DR和DQ抗原表达均高于对照组。虽然单核细胞通常表达这些抗原，但表达增强与激活状态一致。这些结果支持香豆素至少部分通过增强免疫起作用的假说。
