小鼠体内量子点的无创成像。

Noninvasive imaging of quantum dots in mice.

作者信息

Ballou Byron, Lagerholm B Christoffer, Ernst Lauren A, Bruchez Marcel P, Waggoner Alan S

机构信息

Molecular Biosensor and Imaging Center, and Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA.

出版信息

Bioconjug Chem. 2004 Jan-Feb;15(1):79-86. doi: 10.1021/bc034153y.

DOI:10.1021/bc034153y

PMID:14733586

Abstract

Quantum dots having four different surface coatings were tested for use in in vivo imaging. Localization was successfully monitored by fluorescence imaging of living animals, by necropsy, by frozen tissue sections for optical microscopy, and by electron microscopy, on scales ranging from centimeters to nanometers, using only quantum dots for detection. Circulating half-lives were found to be less than 12 min for amphiphilic poly(acrylic acid), short-chain (750 Da) methoxy-PEG or long-chain (3400 Da) carboxy-PEG quantum dots, but approximately 70 min for long-chain (5000 Da) methoxy-PEG quantum dots. Surface coatings also determined the in vivo localization of the quantum dots. Long-term experiments demonstrated that these quantum dots remain fluorescent after at least four months in vivo.

摘要

对具有四种不同表面涂层的量子点进行了体内成像测试。通过活体动物的荧光成像、尸检、光学显微镜下的冷冻组织切片以及电子显微镜，在从厘米到纳米的尺度范围内，仅使用量子点进行检测，成功监测到了量子点的定位。发现两亲性聚丙烯酸、短链（750 Da）甲氧基聚乙二醇或长链（3400 Da）羧基聚乙二醇量子点的循环半衰期小于12分钟，但长链（5000 Da）甲氧基聚乙二醇量子点的循环半衰期约为70分钟。表面涂层还决定了量子点在体内的定位。长期实验表明，这些量子点在体内至少四个月后仍保持荧光。