Zhu Jie, King Tim, Dobrinsky John, Harkness Linda, Ferrier Tricia, Bosma Wim, Schreier Lori L, Guthrie H David, DeSousa Paul, Wilmut Ian
Department of Gene Expression and Development, The Roslin Institute, Midlothian, EH 25 9PS, Scotland, United Kingdom.
Cloning Stem Cells. 2003;5(4):355-65. doi: 10.1089/153623003772032853.
The objective of this study was to evaluate the in vitro and in vivo developmental competence of parthenogenetic (parthenote) pig embryos derived from ovulated and in vitro matured (IVM) oocytes. A total of four experiments were carried out. These demonstrated that the mean blastocyst rates from stimulated ovulated and IVM pig oocytes were not significantly different (61% vs. 46%, p > 0.05) following in vitro culture. Both ovulated and IVM pig parthenotes were able to develop in vivo for 30 days. Parthenote fetuses collected 21 and 30 days post estrus were morphologically normal but significantly smaller and lighter than fertilized controls (p < 0.01). IVM pig parthenotes stopped development around 31 days post estrus.
本研究的目的是评估源自排卵和体外成熟（IVM）卵母细胞的孤雌生殖（孤雌胚）猪胚胎的体外和体内发育能力。总共进行了四项实验。这些实验表明，经体外培养后，刺激排卵和IVM猪卵母细胞的平均囊胚率无显著差异（61%对46%，p>0.05）。排卵和IVM猪孤雌胚均能在体内发育30天。发情后21天和30天收集的孤雌胚胎儿形态正常，但明显小于且轻于受精对照（p<0.01）。IVM猪孤雌胚在发情后约31天停止发育。
