He Gui-jin, Gao Qin-yi, Mo Bin, Dai Xian-wei, Jiang Wei-guo, Sun Duo, Chen Ping-jian
Department of General Surgery, Second Affiliated Hospital, China Medical University, Shenyang 110004, China.
Chin Med J (Engl). 2004 Jan;117(1):104-6.
Internal metallic stents have been widely used in clinical practice, but a high postoperative restenosis rate limits its application. The purpose of this study was to determine the effect of intrabiliary radiation on muscle formation and biliary duct remodeling after biliary duct balloon injury in dogs.
Twenty male dogs (15 - 20 kg) were randomly divided into treatment group (n = 10) and control group (n = 10). Balloon overstretching injury was induced using a balloon catheter placed across the biliary duct. Subsequently, a 103Pd radioactive stent was positioned at the target site in each animal in the treatment group, providing the injured biliary duct with a radiation dose of 12.58 x 10(7) Bq. Dogs in the control group received Ni-Ti stents. All the dogs were killed one month after initial injury. The injured sections were dissected free from the dogs, and were processed for histological and morphological study. Cross-sections were stained with hematoxylin-eosin, Masson's trichrome, and Verhoef-van Giesen. Muscle formation area and lumen area were determined using a computer-assisted image analysis system.
Compared with the control group, 103Pd radioactive stents significantly reduced muscle formation area (78.3%, P < 0.01), and percentage area of stenosis [control stents: (60.0 +/- 21.6)%, 103Pd radioactive stents: (31.6 +/- 9.5)%]. In addition, in the treatment group, the biliary duct lumen area was significantly larger than that in the control group (P < 0.01).
103Pd radioactive stents providing a radioactive dose of 12.58 x 10(7) Bq are effective in reducing muscle formation and biliary duct remodeling after balloon overstretching injury.
金属内支架已在临床实践中广泛应用，但术后再狭窄率较高限制了其应用。本研究的目的是确定胆管内放射对犬胆管球囊损伤后肌肉形成和胆管重塑的影响。
20只雄性犬（体重15 - 20千克）随机分为治疗组（n = 10）和对照组（n = 10）。使用横跨胆管放置的球囊导管造成球囊过度扩张损伤。随后，治疗组的每只动物在靶部位放置一枚103Pd放射性支架，使损伤的胆管接受12.58×10(7)贝克勒尔的放射剂量。对照组的犬接受镍钛合金支架。所有犬在初次损伤后1个月处死。从犬身上取出损伤节段，进行组织学和形态学研究。横断面用苏木精 - 伊红、马森三色染色法和韦尔霍夫 - 范吉森染色法染色。使用计算机辅助图像分析系统测定肌肉形成面积和管腔面积。
与对照组相比，103Pd放射性支架显著减少了肌肉形成面积（78.3%，P < 0.01）以及狭窄面积百分比[对照支架：(60.0 ± 21.6)%，103Pd放射性支架：(31.6 ± 9.5)%]。此外，治疗组的胆管管腔面积显著大于对照组（P < 0.01）。
提供12.58×10(7)贝克勒尔放射剂量的103Pd放射性支架在减少球囊过度扩张损伤后肌肉形成和胆管重塑方面有效。
