He Gui-Jin, Yu Fa-Qiang, Wu Rong, Gao Qin-Yi, Xu Shu-He, Gao Hong, Jiang Wei-Guo, Jiang Tao, Dai Xian-Wei
Department of General Surgery, Second Affiliated Hospital (Shengjing Hospital), China Medical University, Shenyang 110004, China.
Hepatobiliary Pancreat Dis Int. 2007 Oct;6(5):521-6.
With the objective of developing a locally-produced radioactive stent, the present study used in vivo animal experiments to explore apoptosis of proliferative smooth muscle cells resulting from facilitation of the expression of genes caused by gamma-radiation in order to prevent bile duct restenosis. We therefore explored the effects and significance of gamma-radiation on the activity of caspase-3, Fas and Bcl-2 genes in apoptosis of proliferative smooth muscle cells in the bile duct walls of dogs.
Twelve dogs were randomly divided into 2 groups (6 in each group). A postinjury bile duct stenosis model was established and radioactive (103)Pd ((103)palladium) or ordinary bile duct stents were implanted into the bile ducts. HE staining, RT-PCR and immunohistochemistry were used to detect the proliferation and apoptosis of bile duct smooth muscle cells in proliferative endomembrane and the expression of related caspase-3, Bcl-2 and Fas genes.
The expression of caspase-3 and Fas genes in the bile duct tissues of dogs with radioactive stents was higher than that of dogs with ordinary stents. There was significant apoptosis of proliferative smooth muscle cells in the bile ducts. The expression of the Bcl-2 gene in the bile duct tissues of dogs with radioactive stents was lower than that in those with ordinary stents. There was significant apoptosis of proliferative smooth muscle cells in the dogs with low Bcl-2 gene expression.
Radiation increases the activity of caspase-3 and Fas genes and is associated with apoptosis. The radioactive (103)Pd stent may facilitate apoptosis of proliferative smooth muscle cells in the bile ducts of dogs by activating these genes. The Bcl-2 gene expression level is correlated with the occurrence of apoptosis and the radiosusceptibility of cells.
为研发一种本地生产的放射性支架，本研究采用体内动物实验，探讨γ射线辐射促进基因表达导致增殖性平滑肌细胞凋亡，以预防胆管再狭窄。因此，我们探讨了γ射线辐射对犬胆管壁增殖性平滑肌细胞凋亡中caspase-3、Fas和Bcl-2基因活性的影响及意义。
将12只犬随机分为2组（每组6只）。建立损伤后胆管狭窄模型，将放射性（103）Pd（钯-103）或普通胆管支架植入胆管。采用HE染色、RT-PCR和免疫组织化学检测增殖内膜中胆管平滑肌细胞的增殖和凋亡以及相关caspase-3、Bcl-2和Fas基因的表达。
放射性支架犬胆管组织中caspase-3和Fas基因的表达高于普通支架犬。胆管中增殖性平滑肌细胞有明显凋亡。放射性支架犬胆管组织中Bcl-2基因的表达低于普通支架犬。Bcl-2基因表达低的犬中增殖性平滑肌细胞有明显凋亡。
辐射增加caspase-3和Fas基因的活性并与凋亡相关。放射性（103）Pd支架可能通过激活这些基因促进犬胆管中增殖性平滑肌细胞的凋亡。Bcl-2基因表达水平与凋亡的发生及细胞的辐射敏感性相关。
