Usmani Omar S, Biddiscombe Martyn F, Underwood S Richard, Barnes Peter J
Department of Thoracic Medicine, National Heart and Lung Institute, Imperial College London, London, United Kingdom.
J Nucl Med. 2004 Jan;45(1):69-73.
Inhaled radiolabeled aerosols provide invaluable information about in vivo drug deposition. Here, we report our methodology for radiolabeling and imaging monodisperse pharmacologic aerosols in order to study basic aerosol science concepts of drug delivery within the human airways.
We used a spinning-top aerosol generator to produce (99m)Tc-labeled monodisperse albuterol sulfate aerosols of 1.5-, 3-, and 6- micro m mass median aerodynamic diameter.
In vitro Andersen cascade validation data showed that technetium and albuterol were coassociated on each impactor stage for all 3 aerosols, and the radiolabeling process itself did not affect their particle size distributions. Good-quality gamma-camera scintigraphic images of lung and extrathoracic deposition were obtained within an asthmatic patient.
We have successfully radiolabeled and imaged monodisperse albuterol aerosols within the human lungs. This novel technique provides an important tool to relate fundamental concepts of aerosol particle behavior, in vivo deposition, and therapeutic clinical response.
吸入放射性标记气雾剂可提供有关体内药物沉积的宝贵信息。在此，我们报告我们对单分散药理气雾剂进行放射性标记和成像的方法，以研究人类气道内药物递送的基础气雾剂科学概念。
我们使用旋转式气雾剂发生器来制备质量中值空气动力学直径为1.5、3和6微米的（99m）Tc标记的单分散硫酸沙丁胺醇气雾剂。
体外安德森级联验证数据表明，对于所有3种气雾剂，在每个撞击器阶段锝和沙丁胺醇都是共结合的，并且放射性标记过程本身并未影响它们的粒径分布。在一名哮喘患者体内获得了肺部和胸外沉积的高质量γ相机闪烁图像。
我们已成功地对人肺内的单分散沙丁胺醇气雾剂进行了放射性标记和成像。这项新技术提供了一个重要工具，用于关联气雾剂颗粒行为、体内沉积和治疗临床反应的基本概念。
