Dubatolova T, Omelyanchuk L
Laboratory of Cell Cycle Genetics, Institute of Cytology and Genetics, Lavrent'eva 10, Novosibirsk 630090, Russia.
Heredity (Edinb). 2004 Apr;92(4):299-305. doi: 10.1038/sj.hdy.6800408.
Experimental data on spatial and temporal distributions of mosaic clones in Drosophila wing imaginal disc were analyzed. Long-lived proliferation centers (PR1, PR2, and PR3) and areas with decreased proliferation activity were found in the notum region of the disc. Simulation of the growth kinetics of mosaic patches demonstrated that the cell cycle in proliferation centers PR2 and PR3 was shorter than the average cycle in the disc and in the center PR1. A nonrandom clustering of rapidly dividing cells was observed in the PR2, but not in the other cases. The reason why the cell-cycle duration and the clustering of dividing cells may not coincide is discussed in terms of the recruitment of nondividing cells into the cell cycle. The simulation of the time course of the first and second moments of the size distribution of mosaic clones allowed the variance of cell-cycle progression rates to be determined and demonstrated that a model with a continuous cell-cycle rates gave a better fit to the data than the transition probability model of Smith and Martin.
对果蝇翅成虫盘镶嵌克隆的时空分布实验数据进行了分析。在翅成虫盘的背板区域发现了长寿增殖中心（PR1、PR2和PR3）以及增殖活性降低的区域。镶嵌斑块生长动力学的模拟表明，增殖中心PR2和PR3中的细胞周期短于翅成虫盘中的平均周期以及PR1中心的细胞周期。在PR2中观察到快速分裂细胞的非随机聚集，而在其他情况下未观察到。从非分裂细胞进入细胞周期的角度讨论了细胞周期持续时间和分裂细胞聚集可能不一致的原因。镶嵌克隆大小分布的一阶和二阶矩随时间变化过程的模拟使得能够确定细胞周期进程速率的方差，并表明具有连续细胞周期速率的模型比史密斯和马丁的转移概率模型更符合数据。
